Abstract # 207:

Scheduled for Saturday, August 20, 2005 09:00 AM-09:15 AM: Session 15 (Parliament Room) Oral Presentation

Characterization of the genetic structure of a captive chimpanzee (Pan troglodytes) colony

J. Beck1, W. E. Jackson1, R. Hanner1, J. Else2 and J. G. Lorenz1
1Coriell Institute, Coriell Cell Repositories, 403 Haddon Avenue, Camden, NJ 08103, USA, 2Yerkes National Primate Research Center, Emory University, Atlanta, GA
     The genetic structure of a breeding colony of common chimpanzees (Pan troglodytes) located at the Yerkes National Primate Research Center in Atlanta, is being investigated in order to verify familial relationships, determine subspecific status, and recover matrilineal and patrilineal lineage affiliations. A total of 115 individuals from the colony were genotyped at five autosomal str loci, four Y chromosomal str loci, and the mitochondrial cytochrome B, cytochrome oxidase I and HVR-1 loci. The autosomal str data corroborated the assigned parentage for the majority of the colony animals except in one case where both Y chromosome strs and autosomal strs excluded the putative father as being the sire. The comparison of the cytochrome B sequences with sequence data from chimpanzees from Africa show that most of the Yerkes chimpanzees (n = 84) cluster with western African chimpanzees (Pan troglodytes verus) and a minority (n = 10) cluster with central African chimpanzees (Pan troglodytes troglodytes). The familial analysis shows that at least one Yerkes individual is a hybrid between the western and central subspecies. The HVR-1 results corroborate the split between the animals identified as western and central chimpanzees in the cytochrome B analysis. In addition the HVR-1 sequences show linkages among matrilines indicating shared maternal ancestry among various females and certain founding animals for which we have no parentage data are shown to be maternally related to other colony members.