Abstract # 83:

Scheduled for Thursday, August 18, 2005 07:00 PM-09:00 PM: (Cambridge/Oxford Room) Poster Presentation

A rhesus monkey model of influenza infection during pregnancy

G. R. Lubach, C. L. Coe and H. R. Crispen
University of Wisconsin, Harlow Primate Laboratory, Univ. of Wisconsin, 22 N. Charter Street, Madison, WI 53715-1239, USA
     Maternal influenza infections during pregnancy have been implicated in long-term behavioral, neurological, and immunological abnormalities in the offspring. We have developed a rhesus monkey (Macaca mulatta) model to investigate these concerns. Females (n = 10) received intranasal inoculations with live A/Sydney/5/97 influenza virus during the last trimester of pregnancy. Nasal swabs were obtained on Days 3, 7 and 14, post inoculation to assess viral shedding. Blood samples were obtained at baseline, and Days 3, 7 and 14 for antibody levels and other measures. Sham infected females (n = 8) had similar handling. Of the flu-infected females, 87.5% shed virus on Day 3, 62.5% on Day 7, and 0% on Day 14. None of the sham infected shed virus. Additionally, neopterin levels were much higher (P < 0.059) in the inoculated females during infection. At birth, all infants were of normal gestational length and birth weight. At parturition, there was a highly significant positive correlation between maternal and infant IgG antibody to influenza (P < 0.0012). There was no flu antibody in the sham-infected females or their infants. Using factor analysis, preliminary behavioral data indicate infants from flu-infected pregnancies differ significantly (P < 0.05) on two aspects of the Infant Behavioral Assessment Scale: they are poorer at maintaining Directed Attention, and are more difficult to Console after arousal. These data suggest that maternal flu infection during pregnancy impacts fetal development, and may have long-term developmental effects.