Abstract # 52:

Scheduled for Thursday, August 18, 2005 07:00 PM-09:00 PM: (Cambridge/Oxford Room) Poster Presentation

Predicting Follicular Response to Controlled Ovarian Stimulation (COS) in Rhesus Monkeys

J. M. Wu1,2, D. L. Takahashi2, M. S. Lawson2, F. Pau2, J. A. Mattison2, D. K. Ingram3, G. S. Roth3, M. A. Lane4, M. A. Ottinger1 and M. B. Zelinski-Wooten2
1University of Maryland, Dept. of Animal and Avian Sciences, University of Maryland, College Park, MD 20866, USA, 2Oregon National Primate Research Center, Beaverton, OR, 3National Institute on Aging, Baltimore, MD, 4Merck & Co., Rahway, NJ
     Controlled ovarian stimulation (COS) is an alternative to natural breeding, however, these regimens are not available for all primate species. This study evaluated day 3 (d3) follicle-stimulating hormone (FSH) and the clomiphene citrate challenge test (CCCT) in predicting follicular response to COS in rhesus monkeys. Menstrual cycle d3 FSH levels were determined in young (Y; 12 years; n = 8) and old (O; 18-24 years; n = 9) female rhesus monkeys. A CCCT was also performed: serum E2 and FSH were determined on d3 and d10 (menses = d1) and 50mg clomiphene citrate was administered orally d5-9. A normal CCCT yielding d3 and d10 FSH levels <1.0 ng/mL was expected to predict a successful COS. A COS was also performed: Antide, d1-d9; recombinant human FSH (r-hFSH) d1-d6; r-hFSH + r-h luteinizing hormone, d6-d9. A successful COS was determined by the presence of > three 4mm follicles on each ovary and peak E2 levels >1000pg/mL. 8/8 Y and 4/9 O had successful COS. In Y monkeys, d3 FSH (6/8) and CCCT (7/8) were similar in predicting successful COS. In all O monkeys, d3 FSH was >1.0 ng/mL regardless of COS outcome. Conversely, CCCT predicted 1/4 successful and 3/5 unsuccessful COS. Therefore, d3 FSH values <1 ng/mL will be adequate for predicting successful COS. If d3 FSH is >1 ng/mL, however, follow-up with CCCT may aid in identifying animals potentially unresponsive to COS.