Abstract # 1107 Event # 140:

Scheduled for Friday, August 19, 2005 04:00 PM-04:35 PM: Session 12 (Crystal Ballroom) Oral Presentation

Will Functional and Primate Genomics Save the World from Killer Viruses, Pandemics, and Bioterrorism?

M. Katze
University of Washington, Department of Microbiology, University of Washington, Box 358070, Seattle, WA 98195, USA
     We are utilizing Agilent DNA microarrays containing unique human, macaque, and mouse genes and to aid in our bioinformatics effort and to decipher the gene lists we are utilizing Rosetta’s Resolver and Elucidator Software, and Spotfire. It will be critical to examine not only mRNA expression patterns, but also physical levels of cellular proteins, which are altered by virus infection. For our proteomics analysis we are utilizing the Accurate Mass Tag (AMT) approaches, and mass spectrometry to identify and compare complex mixtures of proteins and the novel TAP vector system to perform targeted proteomics. Our laboratory has been utilizing these advances to further our understanding of the effects of virus infection on host cell metabolism, with the intent also of identifying common cellular pathways impacted by unrelated viruses. We will present array and proteomics data from several virus systems including but not limited to Pandemic Influenza, Ebola, West Nile Virus, SARS, SIV, HIV, Hepatitis C Virus, and Vaccinia Virus. We also will describe our Primate Genomics program resources including a description of cDNA libraries, DNA sequencing progress, and Rhesus macaque oligonucleotide microarray development. Finally, a long range goal is to develop a “Virus Compendium” which would enable us to compile a centralized data base to catalogue events in cells infected by all mammalian viruses.