Abstract # 13127 Poster # 99:

Scheduled for Thursday, August 9, 2018 06:00 PM-08:00 PM: (Chula Vista ) Poster Presentation


GLUCOCORTICOID RECEPTORS ARE INCREASED IN THE LAB-HOUSED INTRAUTERINE GROWTH RESTRICTED (IUGR) FETAL BABOON CORPUS CALLOSUM (CC): A POTENTIAL MECHANISM FOR PROGRAMMING OF LATER LIFE CC FUNCTION

H. F. Huber1, J. L. Joganic2, C. Li1,3, D. Xie1, R. Ledevin2, G. D. Clarke4, Y. Heuze2 and P. W. Nathanielsz1,3
1University of Wyoming, 1000 E. University Ave., Laramie, Wyoming 82071, USA, 2Universite de Bordeaux, 3Southwest National Primate Research Center, 4University of Texas Health Science Center San Antonio
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     IUGR causes cognitive deficits. Altered CC shape and size impair cognition and attention. We sought to determine IUGR CC changes for evidence of programming of early aging in baboons (Papio species) born to mothers fed ad libitum (CTL) or 70% global CTL diet (IUGR) in pregnancy and lactation. We used immunohistochemistry to measure term fetal CC glial fibrillary acidic protein (GFAP), glucocorticoid receptor (GR), and phospho-GR (pGR) in IUGR (8 female-F, 9 male-M) and CTL (F13, M13); analysis by t-test of percent area stained. 3T MRI was performed in IUGR (6F, 7M; 5.7 years-y; human equivalent 20y), age-matched CTL (4F, 5M), and a normal life-course baboon cohort (6F, 5M; 12-23y; normal lifespan ~25y). CC was segmented in the midsagittal plane and surface area calculated. A fixed landmark defining the rostrum was identified, and 39 sliding semi-landmarks placed along the resulting outline. Procrustes superimposition and canonical variates analysis were performed. In both sexes, IUGR GFAP decreased, while GR and pGR increased (p<0.05). Adult IUGR females had smaller CC surface areas than age-matched CTL (p=0.04). IUGR male CC shape was more similar to older CTL than age-matched CTL. IUGR CCs were compressed in the midbody, flattened overall with a smaller and more sharply angled rostrum. We conclude IUGR limits CC development, potentially influencing brain function, impairing cognition, and contributing to premature or delayed aging.