Abstract # 13232 Event # 146:

Scheduled for Friday, August 10, 2018 02:30 PM-02:45 PM: (Chula Vista ) Oral Presentation


EVALUATION OF CHOLECALCIFEROL METABOLITES IN CALLITHRIX JACCHUS (COMMON MARMOSET)

A. E. Goodroe, C. Fitz, S. Capuano III and T. E. Ziegler
Wisconsin National Primate Research Center, 1220 Capitol Court, Madison, WI 53715, USA
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     A challenge in maintaining captive common marmosets in an indoor setting is ensuring adequate dietary provision of chlolecalciferol (vitamin D3) due to lack of sunlight exposure. There is variation in dietary D3 provided to research marmoset colonies as dietary requirements have yet to be determined. Evaluation of serum 25-hydroxyvitamin D2&3 (25(OH)D3) in conjunction with the biologically active metabolite 24,25-dihydroxyvitamin D2&3 (24,25(OH)2D3) provides insight into the animal’s ability to hydroxylate D3 into active forms. Our aim was to establish colony reference ranges for 25(OH)D3 and 24,25(OH)2D3, and identify impact of age, sex, and parity as a step towards understanding D3 metabolism and dietary requirements. Serum from 53 marmosets receiving a diet with 8000 IU/ kg D3 was collected. 25(OH)D3 decreased as age increased (ANOVA, p = 0.05) and was lower in multiparous females (T-test, p = 0.05). However, 24,25(OH)2D3 was consistent as was the ratio of 25(OH)D3 to 24,25(OH)2D3. This suggests that although 25(OH)D3 may be low in subpopulations, there is adequate and consistent conversion to 24,25(OH)2D3 in this colony and additional D3 supplementation is not necessary for aged or multiparous marmosets. We evaluated the relationship of serum albumin, calcium, and phosphorous with D3 metabolites; there was no correlation suggesting these values are not adequate biomarkers.