Abstract # 13315 Poster # 169:

Scheduled for Friday, August 23, 2019 06:00 PM-08:00 PM: (Alumni Lounge) Poster Presentation


AGING ASSOCIATES WITH LOSS OF SOMATOSTATINERGIC CELLS BUT INCREASED NUMBER OF ASTROCYTES IN TWO ANIMAL MODELS: CALLITHRIX JACCHUS (COMMON-MARMOSET) AND TUPAIA BELANGERI (TREE-SHREW)

D. Cuervo-Zanatta1, J. Rodríguez-Callejas1, E. Fuchs2 and C. Perez-Cruz1
1CINVESTAV, Av. Instituto Politécnico Nacional 2508, CDMX, CDMX 07360, Mexico, 2German Primate Center, Göttingen, Germany
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     Introduction: Common-marmosets are non-human primates (NHP) spontaneously developing amyloid-beta (AB) plaques and neuroinflammation(1). Tree-shrews are primate-related insectivores that develop AB+ and somatostatin+ (SOM+) plaques in aged brains(2). SOM is a marker of interneurons which decrease in aged animals. Glial fibrillary acidic protein (GFAP) labels astrocytes. Here, we analyzed the number of SOM+ and GFAP+ cells in different brain regions of both species. Methods: Immunofluorescence was used to quantify SOM+ and GFAP+ cells in 3 40 µm brain slices per animal. Young: mean-age 1.75±0.18 (common-marmosets, n=4) and 2.09±0.69 years (tree-shrews, n=4); adult: mean-age 5.33±0.88 (common-marmosets, n=3) and 4.07±0.70 years (tree-shrews, n=6) and aged: mean-age 16.83±2.59 (common-marmosets, n=9) and 7.73±0.21 years (tree-shrews, n=4) animals CA, subiculum and entorhinal cortex brain regions were imaged by confocal microscopy and analyzed by ImageJ. Results: Aged animals had fewer SOM+ cells [ANOVA, alpha=0.001] with increased soma size [ANOVA, alpha=0.05]. GFAP+ cells decreased in hippocampus of aged common-marmosets compared to younger animals [ANOVA, alpha=0.05]. Contrary, increased number of GFAP+ cells with larger soma size was observed in aged tree-shrews [ANOVA, alpha=0.001]. Conclusion: There were differential alterations related to aging and neuroinflammation in both species; however, common-marmosets presented more similarities to human condition(3) and represent an ideal NHP model of aging. References: 1 Rodríguez-Callejas et al. (2016) FN, 8:315; 2 Yamashita et al. (2012) JMP, 41:3; 3Tardif et al., (2019) AJP, 28:2.