Abstract # 13322 Event # 211:

Scheduled for Saturday, August 24, 2019 01:00 PM-01:15 PM: (Room 309) Oral Presentation


G. F. Oliveira, T. E. Ziegler and R. J. Colman
WNPRC, 1220 Capitol Court, Madison, WI. 53715, USA

The alterations induced by social isolation are characteristic of the depressive state in non-human primates. However, cortisol released during stressful events can be ameliorated by oxytocin (OT) release. In order to demonstrate depression-like symptoms we utilize a separation social support test to determine responses to stress at 6 months of age using the Callithrix jacchus (n=26, f=13, m=13). The test began when the animals were placed in a small urine chamber in a room isolated from the marmoset colony. Behavior was recorded in 4x 20-minute blocks and urine collected non-invasively following each 20-minutes. The behaviors: lick, phee call, scent mark, and smell were examined singly and combined to provide a coping rating. Degree of activity was also used as a measure of positive coping. Cortisol and oxytocin were analyzed by Enzyme Immunoassay. We grouped the subjects by basal cortisol levels (>40 v <40 µg/mg creatinine). We found that animals with <40 basal cortisol levels showed higher activity than those with >40 (p= 0.044) prior to isolation. However, coping scores compared to cortisol differences were not significantly different (p>0.999). As we expected, an OT increase accompanied the cortisol increase (p=0.025) with isolation. Hence, social disruption induces stress responses and behavioral coping strategies. These data are important for understanding behavior before comparing with the feasibility of scent-based separation social reward testing. Supported by NIHRO1HD086057.