Abstract # 13324 Event # 120:

Scheduled for Friday, August 23, 2019 11:15 AM-11:30 AM: (Wisconsin Historical Society Auditorium) Symposium


PRACTICAL CONSIDERATIONS TO MAXIMIZE IMMUNOBIOMARKER DATA FOR A BROAD RANGE OF SCIENTIFIC APPLICATIONS

J. L. Yee, K. K. Van Rompay and J. A. Roberts
University of California, Primate Assay Laboratory, California National Primate Research Center, 1 Shields Avenue, Davis, CA 95616, USA
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     Cytokines, chemokines, and other signaling proteins such as interferons, interleukins, and growth factors are secreted by immune system cells. These relatively small molecules facilitate extracellular communication that activates and/or suppresses a wide range of biological functions such as inflammation, proliferation and regulation of the innate and acquired immune system. Although most frequently examined in the context of infectious diseases, these proteins have been shown to be useful biomarkers across a wide range of applications including behavioral, cardiac, metabolic, reproductive, and neuroscience studies. These biomarkers can be particularly valuable in longitudinal studies in nonhuman primates. There are hundreds of these proteins and most have varying functions in multiple, often redundant, pathways. Therein lies both the power and the confusion of analyzing them. The advent of multiplex testing makes it possible to interrogate small samples for a large number of proteins; and more recently, their corresponding RNA. It is essential that the reagents and assays used be well validated for accuracy and reproducibility. This presentation will discuss multiplex microbead immunoassay methods for nonhuman primate testing and consider the roles of species specificity, individual variation, age, sex, viral profile, and sample types in choosing the right assay for the research question and maximizing the generation of valid immuno- and other biomarker data.