Abstract # 13373 Event # 119:

Scheduled for Friday, August 23, 2019 11:00 AM-11:15 AM: (Wisconsin Historical Society Auditorium) Symposium


THE EFFECT OF STRESS HORMONES ON NHP SOCIAL AND NEUROCOGNITIVE DEVELOPMENT.

K. Ethum1,2, Y. Liu1,2, S. Bramlett1,4, E. Morin1,4, D. Guzman1,4, B. Howell1,3,4 and M. Sanchez1,4
1Yerkes National Primate Research Center, Emory University, 954 Gatewood Road, Atlanta, GA 30329, USA, 2Department of Pathology, Emory University, 3Institute of Child Development, University of Minnesota, 4Department of Psychiatry and Behavioral Science, Emory University
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     The Yerkes Biomarkers Core provides liquid chromatography / mass spec (LC/MS) assays for reproductive function, stress physiology, growth, metabolism, circadian physiology, and psychoactive neuropeptides and neurotransmitters in nonhuman primates. The primary LC/MS platform for steroid quantitation in the Core is the AB Sciex Triple Quadrupole 6500 Mass Spectrometer with a Shimadzu chromatography tower. The Core has developed both a corticosteroid as well as a reproductive hormone panel for this platform. Additionally, the corticosteroid panel has been validated for a number of substrates including plasma, CSF, hair, and breast milk. One recent study supported by the Core explores the impact of early life stress (ELS), a known risk factor for psychiatric disorders, on the development of the hypothalamic-pituitary-adrenal (HPA) axis. To test this link, we examined the developmental impact of adverse maternal care on rhesus macaques (Macaca mulatta) HPA axis function longitudinally (birth through adolescence), as measured by plasma cortisol concentrations via LC/MS. We fully characterized HPA axis function in 43 rhesus macaques (21 controls, 22 with ELS experience): (1) baseline activity (diurnal CORT rhythm), (2) stress-induced CORT elevations and (3) glucocorticoid negative feedback via dexamethasone (DEX) suppression tests. Our results show that the Core’s LC/MS CORT is highly sensitive to detect age, sex and experimental group-related differences, not just in stress responses, but in CORT diurnal rhythms, DEX suppression tests and puberty-related CORT changes.