Abstract # 13431 Event # 122:

Scheduled for Friday, August 23, 2019 11:45 AM-12:00 PM: (Wisconsin Historical Society Auditorium) Symposium


NEW TOOLS FOR MULTIPLEX BIOMARKER MEASUREMENT USING NON-INVASIVE AND MINIMALLY INVASIVE SAMPLING

R. Grant1,2, L. Kuller1,2, R. Bellanca1,2, G. Lee1,2 and S. Thompson-Iritani1,2
1Washington National Primate Research Center, University Of Washington, HSB I-421, Seattle, WA 98195, USA, 2University of Washington
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     Analyses of hair and blood samples can be used to measure a number of biomarkers. We couple hair extraction with multiplex hormone and cytokine analysis using a fluorescent multiplex analyte profiling (MAP) to study cortisol as well as other protein biomarkers in hair. Hair cortisol has been used to assess chronic stress in nonhuman primates. Increased hair cortisol has been associated with differences in temperament, alopecia, and cognitive performance in Macaca mulatta, and has been used to measure neuroendocrine activity between Macaca nemestrina dams and their infants. In blood samples we use a multiplex profiling format from NanoString ™ that allows us to test hundreds of potential biomarkers in one assay by measuring differential expression of miRNA, mRNA, and DNA. We have found that specific RNAs change significantly from acute to chronic infection states. Other infectious diseases in macaques may have RNA biomarkers in plasma that will be of diagnostic use. The analysis of miRNA in plasma or serum with Nanostring™ is one new avenue for diagnostics that allow us to screen hundreds of potential biomarkers and validate them more quickly. Using multianalyte profiling techniques with various sample types we are investigating new approaches to biomarker discovery and validation of biomarkers for disease conditions.