Abstract # 2012 Event # 121:

Scheduled for Friday, August 18, 2006 11:10 AM-11:40 AM: Session 11 (Regency East #3) Oral Presentation

Non-invasive Sampling of Mandrill and Drill Monkeys for Use in Genetic Analyses

W. D. Helenbrook, N. S. Wolcott and A. M. McMillan
SUNY College at Buffalo, 1300 Elmwood Avenue, Biology Department, Buffalo, NY 14222, USA
     Limited behavioral, ecological or genetic information exists for the endangered drill monkey, Mandrillus leucophaeus, when compared to its closest relation, the mandrill monkey, M. sphinx. Genotyping analysis utilizing DNA obtained through non-invasively collected fecal samples can provide information about these elusive species but allelic drop-out errors are common when fecal samples yield low quantity and quality DNA. We compared DNA preservation and extraction techniques from 45 fecal samples obtained from five known individual mandrill monkeys at the Buffalo Zoological Gardens. Fecal samples were split into four preservation conditions: air drying, RNAlater®, silica gel beads and the ‘2-step method’ (ETOH then silica gel beads). Preserved samples were split and DNA extracted by Qiagen QIAamp DNA stool kit and Dynabead DNA Direct methods. Preliminary results show RNAlater® and the ‘2-step method’ yielded the highest quantity of DNA, though all preservation methods produced measurable DNA. Qiagen extractions outperformed Dynabeads across all preservation conditions. Allelic drop-out rates are being compared across preservation and extraction methods using five microsatellites we cross-amplified from human STR markers and up to eight other mandrill-specific microsatellites. Drill monkey fecal samples are being collected from wild populations, preserved and extracted based on these results. Mandrill microsatellites will be cross-amplified and used for further genetic studies of both captive and wild drill populations. Supported by ASP Conservation Committee.