Abstract # 64:

Scheduled for Thursday, June 21, 2007 05:00 PM-07:00 PM: Session 7 (South Main Hall) Poster Presentation

Comparisons of rhesus and cynomolgus macaque populations using Single Nucleotide Polymorphisms (SNPs)

S. L. Street1,2, R. Grant3, R. Kyes2, N. Lerche4, H. Wright1, C. Pearson1, R. B. Norgren5 and B. Ferguson1,2
1Oregon National Primate Research Center, 505 NW 185th Ave, Beaverton, OR 97006, USA, 2Washington National Primate Research Center, Seattle, WA 98195, 3SNBL USA, Ltd., Everett, WA 98203, 4California National Primate Research Center, Davis, CA 95616, 5University of Nebraska, Omaha, NE 68198
     Both rhesus macaques (M. mulatta) and cynomolgus macaques (M. fascicularis) are widely used in biomedical research settings. Though sub-population differences in both species can impact study outcomes, few methods are available to compare and validate animal ancestry. Therefore, we studied the genomic variation in rhesus and cynomolgus macaques derived from 6 geographic areas. The 3’ ends of 90 genes were sequenced to identify SNPs in 20 Chinese and Indian rhesus macaques. A subset of those gene regions were also sequenced in cynomolgus macaques from Indonesia, Cambodia, Mauritius and the Philippines. A genotyping assay was then used to survey a collection of 75 cynomolgus and 90 rhesus macaques. The results showed that 1) Indian and Chinese rhesus are distinctly different, sharing only 31% of the 661 SNPs identified in this study 2) Chinese rhesus are a heterogeneous population, containing 70% more SNPs than Indian rhesus 3) half of the rhesus SNPs tested were also polymorphic in cynomolgus macaques 4) Indonesian and Mauritous cynomolgus monkeys are more similar to each other than either are to Cambodian or Philippine animals 5) Cambodian cynomolgus are more similar to Chinese rhesus than are the other cynomolgus macaques. Finally, we demonstrate SNP assays that can distinguish Chinese from Indian rhesus, and Cambodian from Indonesian, Mauritous and Philippine cynomolgus macaques. Supported by NIH/NCRR grants RR00163, RR00166, RR017444.