Abstract # 130:

Scheduled for Friday, June 22, 2007 05:00 PM-07:00 PM: Session 14 (South Main Hall) Poster Presentation

Genetic Assessment of the Macaca nigra at Tangkoko Nature Reserve, North Sulawesi, Indonesia

R. C. Kyes1,2,3,4, D. I. Paulsen1,2, D. Perwitasari-Farajallah3, L. Jones-Engel2, J. Onibala4, E. Iskandar3 and B. Ferguson2,5
1Department of Psychology, University of Washington, Seattle, WA 98195, USA, 2Washington National Primate Research Center, University of Washington, 3Primate Research Center, Bogor Agricultural University, Bogor, Indonesia, 4Faculty of Animal Sciences, Sam Ratulangi University, Manado, North Sulawesi, Indonesia, 5Oregon National Primate Research Center
     The Sulawesi black macaque (Macaca nigra) population at the Tangkoko Nature Reserve in North Sulawesi, Indonesia has been the focus of periodic assessment since the late 1970s. Although past surveys have shown ongoing decline, recent data indicate a relatively stable population (Kyes et al., 2004). Currently, we are expanding our research approach to include genetic assessment of the population. DNA isolated from fecal samples from three groups was used to amplify the Cytochrome B locus of mitochondrial DNA. We successfully amplified the 200 bp region and sequence analysis demonstrated a match to a previously derived M. nigra CytoB sequence (via whole blood, Kyes, et al., 2004), indicating that the extracted samples were a valid source of M. nigra DNA. A few of the CytoB sequences identified 1 or 2 nucleotide substitutions, confirming that the fecal samples were derived from multiple animals. The extracted DNA samples were not adequate for the PCR amplification of single copy sequence, and thus whole genome amplification was used to increase the DNA content. The resulting samples were analyzed for both mitochondrial and single copy sequence variation. Finally, the SRY locus, located on the Y chromosome, was tested and found useful for distinguishing male and female M. nigra samples. Subsequent analysis is underway to assess genetic diversity using STR loci. Supported in part by NIH Grant RR-00166 and Woodland Park Zoo.