Abstract # 132:

Scheduled for Friday, June 22, 2007 05:00 PM-07:00 PM: Session 14 (South Main Hall) Poster Presentation

Use of Next Generation Sequencing Technologies for Generating Non-Human Primate Genomic Resources

W. Courtney1, S. McGrath1, D. Locke1, V. Magrini1, M. Hickenbotham1, J. Armstrong1, A. Quinlan2, G. Marth2 and E. R. Mardis1
1Washington University School of Medicine, Genome Sequencing Center, 4444 Forest Park Blvd., St. Louis, MO 63108, USA, 2Boston College, Department of Biology
     The availability of genomic sequence data for non-human primates such as the rhesus macaque and common marmoset represents an initial resource that will transform research that utilizes these animals as models of human disease. We now have begun to apply the power of next generation sequencing technologies, coupled with carefully chosen genomic DNA samples, to rapidly and cheaply generate important resources that augment the genome sequence assemblies. However, the value of these resources to the user community requires their input—both scientific (what resources are needed?) as well as samples (which animals should be utilized?). These resources will include genome-wide SNP and miRNA discovery, tissue-specific, normalized, full-length cDNA (including splice variant detection) sequencing. Our approaches utilize both the 454 Life Sciences’ FLX pyrosequencer (cDNA sequencing) and the Solexa sequencer (SNP and miRNA discovery). In one collaboration, we already have interacted with the common marmoset community to identify individuals suitable for genome-wide SNP discovery. Once SNPs have been placed across the genome sequence, for example, inexpensive genotyping arrays can be generated such that each animal entering a study can be genotyped, and results of the study can be interpreted in that context. A detailed description of a web-based browser specifically developed to display these resources in the context of human genome annotations will feature SNP discovery for the rhesus macaque and marmoset genomes.