Abstract # 35:

Scheduled for Thursday, June 19, 2008 05:00 PM-07:00 PM: Session 4 (Ball Rooms A and B) Poster Presentation

Genetic Assessment of the Tibetan Macaques (Macaca thibetana) at Huangshan National Reserve, Anhui, China

D. Xia1, J. Li1, R. C. Kyes2, Y. Zhu1, S. Street3 and B. Ferguson3
1Anhui University, School of Life Sciences, Hefei, Anhui, China, 2Dept of Psychology & Washington National Primate Research Center, University of Washington, Seattle, WA, 3Oregon National Primate Research Center, Beaverton, OR
     Huangshan National Reserve in Anhui Province, China, is a World Culture and Nature Heritage site that is well-known as a tourist destination and research site for the study of Tibetan macaques (Macaca thibetana). Two habituated groups of macaques (YA1 and YA2) living in the reserve have been studied for more than 15 years. However, very little is known about the status of the reserve’s remaining macaque populations. During a recent survey (Nov-Dec 2007), we encountered at least one new group of macaques (Grp 3) and were able to obtain the fecal samples for analysis of genetic diversity. DNA isolated from 21 fecal samples from three groups (YA1, YA2, Grp3) was used to amplify a 390 bp region of the 12S rRNA locus in the mitochondrial DNA. Preliminary results from the sequence analysis indicated that all samples were from the predicted 12S rRNA region. The samples most closely matched the sequences of M. assamensis and M. thibetana (Tosi et al., 2003) sharing 97% and 96% sequence identity respectively. None of the DNA sequences were completely identical to previous published M. assamensis and M. thibetana sequences. Further analysis is underway to examine microsatellite heterozygosity to address genetic diversity within and among groups. The research was supported in part by the Natural Science Foundation of China (NSFC), Woodland Park Zoo, One Earth Institute, and NIH Grants RR-00166 and RR00163.