Abstract # 148:

Scheduled for Sunday, September 20, 2009 06:30 PM-09:00 PM: Session 14 (Mission Bay Ballroom CDE) Poster Presentation


PLATYRRHINE (NEW WORLD MONKEY) PHYLOGENY BASED ON WHOLE GENOME SHOTGUN LIBRARIES

N. M. Jameson1, S. V. Yi2, J. C. Opazo1,3 and D. E. Wildman1,4
1Wayne State University School of medicine, Center for molecular medicine and genetics, 540 E. Canfield Ave, Detroit , MI 48201, USA, 2School of Biology, Institute of Bioscience and Bioengineering, Institute of Biosystems, Georgia Institute of Technology, Atlanta, GA 30332, USA, 3 Current Address: Instituto de Ecologia y Evolucion, Facultad de Ciencias,Universidad Austral de Chile, Casilla 567, Valdivia, Chile., 4Department of Obstetrics and Gynecology, Wayne State University School of Medicine and Hutzel Women's Hospital, Detroit, MI 48201
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     The phylogenetic relationships of neotropical primates have been extensively studied from a molecular perspective, yet key questions remain. Here we provide a framework for using non-genic, non-coding markers in comparative primate phylogenomic studies in species whose genomes are not yet scheduled for complete sequencing. The utility of such markers is that they are relatively free from the constraints imposed by natural selection. A random genomic shotgun library was generated from the Gray-bellied Night monkey Aotus lemurinus. Eleven unlinked, non-coding, non-genic, non-repetitive, nuclear DNA markers derived from the Aotus library were sequenced in at least one representative species of every platyrrhine genus. The combined sequence from these markers resulted in an ~8 kb multiple sequence alignment of 22 taxa. All analyses converged on a single topology for the platyrrhine generic relationships. Notably, we confirmed that Pitheciidae is sister to the other two platyrrhine families (Cebidae, Atelidae). This relationship is supported by high branch support values and topology tests. Additionally, within the Cebidae, we inferred the phylogenetic position of Aotus as a sister genus to the remaining cebids. With this well-resolved genus level phylogeny in place it is now possible to design and test hypotheses regarding the evolution and diversification of platyrrhine phenotypes, behaviors, and life histories. This research was funded by National Science Foundation grants (BCS-0751508) to DW and (BCS-0751481) to SY.