Abstract # 191:

Scheduled for Sunday, September 18, 2011 07:00 PM-09:00 PM: Session 23 (Salon G (Sixth Floor)) Poster Presentation


N. D. Simons1, J. G. Lorenz1, L. K. Sheeran1, M. D. Matheson1, J. Li2 and R. S. Wagner1
1Central Washington University, Dean Hall 334, Ellensburg, WA 98926, USA, 2Anhui University, Hefei, People's Republic of CHina
     Cryptic and endangered fauna, including many primate taxa, pose challenges when it comes to the noninvasive collection of their biomaterials. For this reason the application of noninvasive genotyping to primates has been limited to the use of low quality samples (i.e. degraded DNA). We present a successful method for the noninvasive collection of saliva from habituated, free-ranging monkeys. An apparatus was designed with the goal of collecting saliva from individual, identifiable monkeys. The method and apparatus were used in the collection and successful extraction and amplification of the Cytochrome B and MHC-DR Beta 1 genes (used in phylogenetic and kinship studies) in 18 individuals from a population of Tibetan macaques (Macaca thibetana) in the Valley of Wild Monkeys in Huangshan, People’s Republic of China. This sample size (n=18) represents 60% of the Yulingkeng 1 (YA1; n=30) population, and includes juvenile, sub-adult and adult individuals of both sexes. These results indicate this is an effective technique for the noninvasive collection of saliva across age/sex class in a free-ranging, terrestrial primate species. The collection of high-quality saliva samples from individuals in free-ranging primate populations could have a wide-range of implications for epidemiological studies, hormonal analyses of HPA Axis functioning, pathogen screening, non-invasive genotyping, and behavioral genetics.