Abstract # 44:

Scheduled for Saturday, September 17, 2011 01:55 PM-02:15 PM: Session 10 (Salon F (Sixth Floor)) Oral Presentation


SNP PANELS FOR GENOTYPING CYNOMOLGUS MACAQUES (MACACA FASCICULARIS)

D. Smith1, P. Houghton2, S. Kanthaswamy1, D. A. George1, J. A. Satkoski Trask1 and A. Premathusan1
1University of California, Davis, Department of Anthropology, One Shields Avenue, Davis, CA 95616, USA, 2Primate Products, Inc.
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Cynomolgus macaques are one of the two most frequently used primates in biomedical research. As for their close relatives, the rhesus macaque, regional populations of the species exhibit marked genetic and clinical differences that influence their suitability as animal models. However, the geographic region of origin of most cynomolgus macaques imported for biomedical research is not well documented. Thus, in addition to using genetic markers to identify/confirm the parentage of each animal and uniquely characterize each animal, genetic methods to identify geographic region of origin are also needed. In addition, cynomolgus macaques from Indochina have variably, but unknown levels of admixture with rhesus macaques which might influence their candidacy as subjects in research. Other genetic characteristics, such as MHC and ABO blood group phenotypes are also needed to select animals appropriate for infectious disease and transplantation research. We have developed SNaPshot assays and highly informative SNPs for identifying parentage and estimating kinship between animals, geographic region of origin, proportion of rhesus macaque ancestry in Indochinese cynomolgus macaques, genotypes involving 9 MHC alleles and the ABO blood group phenotype.