Abstract # 119:

Scheduled for Friday, June 22, 2012 02:00 PM-02:15 PM: Session 19 (3rd Floor All Space)


THE OUHSC SPF BABOON (PAPIO HAMADRYAS ANUBIS) COLONY MAINTAINS HIGH DEGREE OF GENETIC DIVERSITY.

J. J. Ely1, A. Gillapsy 2, J. F. Papin3, G. White3 and R. Wolf3
1Alamogordo Primate Facility, Bldg. 1303, POB 956, Holloman AFB, NM 88330-0956, USA, 2University of Oklahoma Health Science Center, Laboratory for Molecular Biology and Cytometry Research, Oklahoma City, OK, 3University of Oklahoma Health Science Center, Department of Comparative Medicine, Oklahoma City, OK
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     The long-term health of isolated captive populations dictate that genetic diversity among population founders should be representative of wild populations, and should be maintained across generations of captive breeding. Conventional colony founders were originally obtained from diverse, presumably independent sources, including many drawn from wild African populations. We assessed genetic diversity among founders of the OUHSC SPF baboon colony by selection of 30 SPF founders and Sanger sequenced a 342 bp fragment of the mtDNA HVR1. Data were analyzed with MEGA 4.0. We identified 22 unique haplotypes, 53 segregating nucleotide sites, a nucleotide diversity ?=0.027 and mean genetic distance D=0.029. By contrast, African P. h. anubis baboons had only 4 unique haplotypes in 18 homologous sequences, with only 4 segregating sites and a mean genetic distance D=0.006. Unlike African anubis, phylogenetic analysis revealed no evidence of hybridization with P.h. hamadryas in SPF founders. Assessment of 14 microsatellite loci revealed 8.6 alleles/locus in the SPF population and 74% mean heterozygosity. Both measures were virtually unchanged from initial estimates in the conventional colony several generations back (8.6 and 76%, respectively). This indicated that there was no inadvertent population bottleneck during selection of SPF founders. Results demonstrated that initial founder genetic diversity was higher that found among wild anubis. Furthermore, prudent genetic management has successfully maintained the initial high degree of founder genetic variation among the population descendants.