Abstract # 4493 Poster # 78:

Scheduled for Thursday, June 20, 2013 07:00 PM-09:00 PM: Session 9 (SG Foyer ABC) Poster Presentation


POLYMORPHISM IN THE OPRM1 GENE AMONG OLD WORLD MONKEYS

S. G. Lindell, J. G. Clemente and C. S. Barr
NIH/NIAAA/LNG/SCBG, 5625 Fishers Lane, Rockville, MD 20852, USA
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     Variation in the first exon of the mu-opioid receptor gene (OPRM1) exists in humans and macaques. Both variants cause amino acid changes that moderate reward and stress response and appear to be under selection. We wanted to determine whether interspecific variation at OPRM1 occurs among Old World monkeys (OWMs). Individuals from nine genera within Cercopthecidae (OWMs) were sequenced. Five of the genera were from the Cercopthecini tribe (Allenopithecus, Miopithecus, Erythrocebus, Chlorocebus, and Cercopithecus, N=14). Nine species of macaque along with three other genera of Cercopithecidae [Papionini tribe (Lophocebus, Papio, Macaca) and Colobinae subfamily (Colobus)] were also sequenced (N=38). Publically (UCSC) available sequence was used for comparison to a New World monkey (Saimiri). We detected allelic variation (Chr4:111175813) that predicts a non-neutral amino acid substitution in the OPRM1 ligand-binding domain (Asp12>Lys12). All species from Cercopthecini were homozygous for the C allele at this position, while all others (including Saimiri) were homozygous for the T allele. Whether due to genetic drift or to natural selection, our data suggest the possibility that an OPRM1 variant arose and went to fixation after the divergence of Cercopthecini from Papionini.