Abstract # 4536 Poster # 89:

Scheduled for Thursday, June 20, 2013 07:00 PM-09:00 PM: Session 9 (SG Foyer ABC) Poster Presentation


VASOPRESSIN 1A RECEPTOR BINDING SITES IN THE BRAIN OF THE SOCIALLY MONOGAMOUS TITI MONKEY (CALLICEBUS CUPREUS)

S. M. Freeman1, L. J. Young1 and K. L. Bales2,3
1Emory University, Yerkes National Primate Research Center, Center for Translational Social Neuroscience, 954 Gatewood Rd., Atlanta, GA 30329, USA, 2University of California- Davis, 3California National Primate Research Center
line
     The coppery titi monkey (Callicebus cupreus) has been used to investigate the behavioral neuroendocrinology of primate pair-bonding, and the neuropeptide hormone vasopressin (AVP) has been shown to influence this species-typical social behavior in titi monkeys. However, the distribution of the vasopressin 1a receptor (V1aR) in the titi monkey brain is not yet known. Because the available radioligand for V1aR receptor autoradiography also has a modest affinity for the oxytocin receptor (OTR) in primates, we used a pharmacologically informed competitive binding receptor autoradiography protocol to selectively reveal V1aR. Specifically, the V1aR radioligand (125I-LVA) was incubated on adjacent tissue sections in one of three conditions: 50 pM radioligand alone, or in the presence of 10 nM SR49059 (a selective human V1aR ligand), or 20 nM ALS-II-69 (a selective human OTR ligand). The resulting V1aR binding distribution is widespread throughout the brain, including: cortex, nucleus accumbens, arcuate nucleus, caudate, putamen, hippocampus, amygdala, thalamic nuclei, cerebellum, and choroid plexus. Furthermore, we show that SR49059 drastically reduces V1aR binding in the titi monkey brain. Because SR49059 is an orally active, small molecule V1aR antagonist, this compound emerges as an excellent candidate for pharmacological manipulation of V1aR in behavioral experiments in titi monkeys. Funding: MH090776, MH64692 to LJY; NIH HD053555, Office of Research Infrastructure Programs, and the Good Nature Institute to KLB; P51OD11132 to YNPRC; P51OD011107 to CNPRC.