Abstract # 4570 Poster # 183:

Scheduled for Friday, June 21, 2013 07:00 PM-09:00 PM: Session 21 (SG Foyer ABC) Poster Presentation


VALIDATING THE USE OF URINARY C-PEPTIDE AS A MEASURE OF METABOLIZABLE ENERGY INTAKE IN CAPTIVE COLOBUS MONKEYS – A VARIATION ON PRESERVATION METHODS.

L. C. Loyola, L. P. Turcotte and R. A. Delgado
University of Southern California, 3616 Trousdale Pkwy, AHF 107, Department of Biological Sciences, Los Angeles, CA 90089, USA
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     Previous studies have utilized the measure of urinary C-peptide to quantify energy balance in apes, and as a means to determine the energetic profiles of various catarrhine primates. This project aimed to validate preservation and extraction methods previously developed with ape urine samples for use with colobine samples and to calculate urinary c-peptide (UCP) levels as a measure of metabolizable energy intake (MEI). We collected 8 samples from adult male and female black & white colobus monkeys (Colobus guereza) from the Los Angeles Zoo from November 2011 to January 2012. Prior studies on wild colobus populations froze urine samples for preservation; however, we dried 200uL aliquots onto filter paper, a method previously used with ape urine. Samples were stored in a dry, sealed container for 2 weeks. They were reconstituted and analyzed via enzyme-linked immunosorbent assay (ELISA, sensitivity = 0.2ng/ml) to quantify C-peptide and colorimetry assay to measure creatinine levels. We found that the male did not vary from female UCP concentrations. However, as in previous studies, the lactating female had a higher UCP concentration than the other two females. This study demonstrates that proposed preservation methods for urine collected from wild colobus populations, and the use of UCP as a reliable measure of MEI, are valid for field studies on the energetic profiles of additional primate taxa beyond apes.