Abstract # 4609 Poster # 161:

Scheduled for Friday, June 21, 2013 07:00 PM-09:00 PM: Session 21 (SG Foyer ABC) Poster Presentation


THE RHESUS MACAQUE AS A MODEL FOR THE STUDY OF ENDOMETRIOSIS

L. A. Ruiz1, I. Flores2, O. D. Gonzalez1, A. G. Burgos1, M. Arce1, A. T. Fazleabas3, E. Kraiselburd1 and J. Gonzalez1
1Caribbean Primate Research Center (CPRC), University of Puerto Rico (UPR), P.O. Box 1053, Sabana Seca 00952, USA, 2Ponce School of Medicine & Health Sciences, 3Michigan State University
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Endometriosis causes incapacitating pelvic pain and infertility to 1 in 10 women of reproductive age. Still today its etiology is unknown. We proposed to use the rhesus macaque, Macaca mulatta, as an animal model of endometriosis and to validate its use to pursue future studies on the etiology, treatment and prognosis of this disease. AIMS: 1) to identify spontaneous endometriosis cases in our colony and 2) to induce endometriosis experimentally.  This study was reviewed and approved by institutional IACUC. The endometriosis was induced with inoculation of autologous menstrual tissue performed during three menses into the peritoneal cavity by laparoscopy (n=5). Identification of animals with spontaneous endometriosis was observed by laparoscopy that was performed during the selection of subjects and at necropsy. cDNA microarray analysis of gene expression was performed in lesions from animals with spontaneous endometriosis.  We identified 17 cases of spontaneous endometriosis confirmed by a veterinary pathologist. Pedigree and Kinship analysis demonstrated familial aggregation. In one monkey in which endometriosis was induced, one lesion was observed one month after the inoculations. Candidate genes were identified by microarray analysis. This study revealed that monkeys with spontaneous endometriosis might have a genetic component. These results support the great potential of the UPR Primate Center to become a valuable resource for endometriosis research. Supported by NIH-PRTCRC #8U54MD007587-03 and NIH/ORIP-CPRC #P40 0D012217.