Abstract # 122:

Scheduled for Friday, June 21, 2013 12:05 PM-12:20 PM: Session 14 (San Geronimo Ballroom C) Oral Presentation


GENETIC DIVERGENCE BETWEEN TWO ALLEGED SUBSPECIES OF CYNOMOLGUS MACAQUES IN THE PHILIPPINES

S. Kanthaswamy1, P. Houghton2, J. Ng1, D. George1, J. Satkoski1 and D. G. Smith1
1Molecular Anthropology Laboratory, Department of Anthropology, Young Hall, UC Davis, Davis, CA, , Department of Anthropology, Young Hall, UC Davis, Davis, CA 95616, USA, 2Primate Products International
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     Philippine cynomolgus macaques (Macaca fascicularis) were assigned by Fooden (1991) to two different subspecies based on geography and pelage color, both of which are employed as subjects in biomedical research. M. f. philippinensis inhabits Luzon and the Palawan Islands and exhibits dark dorsal pelage. M. f. fascicularis is restricted to SW Mindanoa and the Sulu Archipelago and exhibits pale dorsal pelage like cynomolgus macaques in Malaysia and Indonesia. Both alleged subspecies probably reached the Philippines from Sabah by two different routes but not necessarily at the same time. We genotyped 30-40 cynomolgus macaques each from southern Luzon, the western portion of SW Mindanoa, Sabah and southern Sumatra for 16 short tandem repeat (STR) loci and sequenced an 835 bp fragment of mitochondrial DNA (mtDNA) including the HVSI region. Principal coordinates (PCA) and STRUCTURE analyses were performed to assess genetic relationships based on STRs and a median joining network (MJN) was constructed from the mtDNA sequences. The PCA, STRUCTURE plot and MJN supported the existence of only two populations with all samples from Sabah and Sumatra in one group and both Philippine populations in the other. The mtDNA sequences in the two groups were predominantly ancestral and derived, respectively. Since these results are inconsistent with Fooden’s hypothesis, Philippine cynomolgus macaques should be regarded as a single homogeneous animal model for the study of human disease.