Abstract # 5859 Poster # 71:

Scheduled for Saturday, September 13, 2014 07:00 PM-09:00 PM: Session 11 (Decatur B) Poster Presentation


MU AND KAPPA OPIOID RECEPTOR BINDING IN THE FOREBRAIN OF THE MONOGAMOUS TITI MONKEY (CALLICEBUS CUPREUS)

B. J. Ragen1,2, S. M. Freeman2, S. A. Laredo1,3, S. P. Mendoza2 and K. L. Bales1,2,3
1University of California, Davis, Department of Psychology, One Shields Ave, Davis, CA 95616, USA, 2California National Primate Research Center, Davis, CA 95616, 3University of California, Davis, Animal Behavior Graduate Group, Davis, CA, 95616
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     The opioid system is involved in infant-mother attachment as well as pair-bonding in adults. Mu opioid receptor (MOR) manipulation in the monogamous titi monkey alters cortisol and vasopressin levels, and the presence of a pair-mate ameliorates the negative effects of opioid blockade. Furthermore, the kappa opioid receptor (KOR) aids in pair-bond maintenance in titi monkeys and the monogamous prairie vole. The goal of the present study was to map MOR and KOR binding in the titi monkey. The brains of 9 adult titi monkeys (N=3 paired males; N=3 paired females; N=3 unpaired males) were removed and flash frozen. Autoradiography was performed using [3H]DAMGO to examine MOR binding and [3H]U69,593 to examine KOR binding. Tissue used for MOR and KOR binding was exposed to film for 21 weeks. Strong MOR binding was found in the nucleus accumbens, medial amygdala, medial septum, paraventricular nucleus of the hypothalamus, and mediodorsal thalamus. Binding was in other amygdalar nuclei, hypothalamic nuclei, bed nucleus of the stria terminalis, lateral septum, diagonal band, hippocampus, dorsal striatum, and cingulate gyrus. KOR binding was found in the claustrum, amygdala, hippocampus, and cortex with light binding in the striatum. These data provide information on where pharmacological manipulations are acting and which opioid-dependent brain areas may be involved in pair-bonding. Funding: Good Nature Institute; NICHD: HD053555; Office of Research Infrastructure Programs: Grant P51OD01107.