Abstract # 7890 Event # 187:

Scheduled for Monday, August 28, 2017 10:00 AM-10:20 AM: (Grand Ballroom) Oral Presentation


HIGH-RESOLUTION METABOLOMICS TO MONITOR METABOLIC RESILIENCE IN MARMOSET AGING

D. Jones1, A. Todor1, S. Li1, S. D. Tardif2, J. F. Hoffman3 and D. E. Promislow4
1Emory University, Dept of Medicine, Atlanta, GA 30322, USA, 2Southwest National Primate Research Center, 3University of Alabama, 4University of Washington
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     We previously reported cross sectional and longitudinal changes associated with aging for plasma metabolomics in 200 marmosets collected over 5 years. We have initiated studies of metabolic network structures to determine whether these can be used to evaluate changes in metabolic resilience with aging. The central concept is that metabolic adaptability depends upon the ability of a system to adjust to stresses; a more irregular state with many connections has better ability to tolerate stress. We selected metabolic networks of methionine (Met) for initial study because Met is an essential amino acid readily measured in all marmosets and prior studies in rodents showed that Met restriction can promote lifespan. We used pathway connections and network diameter as indicators of resilience. Results comparing 32 females >7 years old to 74 females <7 years old showed decreased Met pathway connections and greater network diameters for older marmosets (p<0.05). A similar pattern was seen in males but results were not significant. In a cross-over dietary study (n=8), a diet with low Met resulted in greater connectivity and decreased network diameter compared to high Met diet. These results show that network diameter varied as expected in association with age and improved diet and provide a foundation for experimental studies to evaluate interventions to improve resilience in aging.Research Support by National Institute of Aging (AG038746).