Abstract # 8017 Poster # 42:

Scheduled for Saturday, August 26, 2017 06:00 PM-08:00 PM: (National Ballroom AB) Poster Presentation


EVOLUTION OF CYP2C GENE CLUSTER AMONG THE HOMINOIDEA

M. E. Chaney1, A. J. Tosi1 and H. Piontkivska2
1Kent State University, Department of Anthropology, 226 Lowry Hall, Kent, OH 44242, USA, 2Kent State University, Department of Biological Sciences, 256 Cunningham Hall, Kent, OH 44242
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     Cytochrome P450 enzymes are encoded by a diverse superfamily of genes, some of which detoxify so-called xenobiotic compounds from an animal’s diet (e.g., plant defensive compounds). Genes from this functional category have been shown to be prone to duplication, pseudogenization and/or deletion. Our study examines the CYP2C subfamily of genes, which are all present in a similar syntenic pattern among well-annotated anthropoid genomes. We aligned genomic DNA and mRNA sequences for all syntenic protein-coding CYP2C genes available for Homo sapiens, Pan troglodytes, P. paniscus, Gorilla gorilla, Pongo abelii, Nomascus leucogenys, and Macaca mulatta in the NCBI Gene and Ensembl 87 databases. An intron shared by 26 out of 30 protein-coding CYP2C genes (range: 3-9 genes per species) was analyzed by maximum likelihood (ML); this analysis revealed close phylogenetic cohesion of these genes within four gene-clades. Additional ML analyses of the translated protein-coding sequences (28 of 30 genes) implied putative cohesion of function among these genes as well. Overall, our data indicate an instance of retained duplication of the CYP2C9 gene in the basal hominoid lineage, independent deletion of the CYP2C18 gene in the panin lineage and that of Nomascus leucogenys. Because these genes encode enzymes that interact with exogenous substrates present in the diet, our results provide insights for further inferences of the early ecology or physiology of the hominoid radiation.