Abstract # 127:

Scheduled for Saturday, August 26, 2017 04:00 PM-04:15 PM: (Grand Ballroom) Oral Presentation


E. K. Hutchinson, S. P. Flemming and C. M. Garrett
Johns Hopkins University, 720 Rutland Ave, Ross 459, Baltimore, MD 21224, USA
     Two adult female common marmosets at Johns Hopkins University presented for chronic anorexia following stressful events. One animal refused to eat following social separation from her mother and then her human handler. The animal weighed 280g when we initiated therapy with midazolam, a short-acting benzodiazepine anxiolytic, given in conjunction with twice daily meal times. After dose titration with minimal voluntary food consumption, on d8 a dose of 0.2 mg/kg was administered intramuscularly, at which time the animal consumed all of her 30g food ration. Using this same dose over the next 20 days, the animal consumed >75% of her ration for all but two meals when given midazolam, still refusing to eat when offered food without midazolam treatment. Over this time her body weight increased to 318g, and we observed that the delay between injection and first consumption of food had decreased. This prompted us on d20 to attempt therapy with saline only, after which the animal readily consumed her total ration. This result was repeated with the same result, which we attribute to a learned association (via classical conditioning) between the injection procedure and increased appetite. It remains unclear whether this effect on anorexia is due to anxiolysis or direct appetite stimulation, but we have produced and will present similar results using oral midazolam in this same and a second animal.