Abstract # 148:

Scheduled for Friday, August 19, 2005 07:00 PM-09:00 PM: (Cambridge/Oxford Room) Poster Presentation


M. L. Sparman, C. M. Ramsey, C. M. Thomas, S. M. Mitalipov, J. W. Fanton, G. M. Maginnis, R. L. Stouffer and D. P. Wolf
Oregon National Primate Research Center, Div. of Reproductive Sciences, 505 NW 185th Ave., Beaverton, OR 97006, USA
     The Caribbean vervet was evaluated as a model for reproductive research based on general health, availability, cost, lack of seasonal anovulation, straight cervix, and human-like sperm characteristics. Eight adult female vervets were hormonally monitored for their potential use as egg donors. The six females displaying regular menstrual cycles were subjected to controlled ovarian stimulation involving human recombinant gonadotropins. Three animals failed to respond. Laparoscopic follicular aspiration was performed on the other three females at 27-30 h post-hCG administration. A total of 62, 40, and 18 oocytes were recovered of which 30, 20, and 4, respectively, were at the metaphase II stage and inseminated using intracytoplasmic sperm injection. Sixty, 10 and 50 percent of the inseminated oocytes were fertilized based on pronuclear formation and cleavage. Embryos from two of the three stimulated females developed into expanded blastocysts. Cervical instrumentation was problematic. Two adult male vervets were assessed as sperm donors. Neither adjusted well to the restraint and collection procedure required for penile electroejaculation. Sperm collected via rectal electroejaculation resulted in samples with very low sperm motility and concentration. Caudal epididymal aspirations from one male yielded an adequate concentration of motile sperm (231 million sperm/mL; 32% motility). These results emphasize the need to establish species-specific ovarian stimulation protocols and semen collection techniques if vervets are to be considered as an alternative research model. RR00163