Abstract # 51:

Scheduled for Thursday, August 18, 2005 07:00 PM-09:00 PM: (Cambridge/Oxford Room) Poster Presentation

Analysis of factors influencing success in the derivation of Specific Pathogen Free (SPF) Rhesus Macaque (Macaca mulatta) from Herpes B and STLV positive breeding groups

I. D. Lussier, S. Howell and G. C. Westergaard
Alpha Genesis Inc., 95 Castle Hall Rd, Yemassee, South Carolina 29945, USA
     Demands for Herpes B and simian T-cell Leukemia Virus (STLV) negative macaques have risen dramatically in recent years, and far exceed production from established SPF colonies. In this environment it is crucial to maximize production to meet demand. Over the last decade, SPF colonies of rhesus macaques (Macaca mulatta) have been established at several primate centers, often utilizing virus positive breeders from which young virus free animals are removed. In this study, we examined factors that influence success in SPF offspring derivation from non-SPF rhesus macaque breeding groups to refine derivation methods for optimal success. Factors included age of separation from natal group, sex, and screening time period for 122 offspring (59:63). Derivation screenings were conducted via blood sampling at three month intervals across a one year period and ELISA tested for the presence of the B and STLV viral agent. Results indicate that 92% of offspring were successfully derived. Of the remaining offspring, 3.3% had seroconverted while still in natal group, 3.4% seroconverted between removal from natal group (pre-screening) and the first screening, and 1.7% showed virological activity at the second screening. Results indicate age of separation from the natal group was the factor that best predicted seroconvertion rates and derivation success. Results suggest separating infants prior to 9.6 months is the best way to predict viral status. This research was supported by NIH grant number 5U01AI49916.